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l shot glass
l regular glass
l coffee mug
l cigarette lighter
l X-Acto knife or sharp steak knife
l microwave oven
Several things need to be accomplished. First, we need to sterilize a
shot glass to easily mix the spore solution and we need to sterilize a
syringe to hold the solution. We also need some sterile water in which
to suspend the spores. The following procedure will accomplish all of
this.
Fill a coffee mug with water and place a shot glass inside the coffee
mug. Make sure the shot glass is completely submerged. Place the
coffee mug in the microwave oven and get the water to a full boil for
10 minutes. It does not need to be a violent boil. Adjust the heat level
of the microwave oven to keep too much water from being lost if
necessary.
Remove the shot glass and empty the excess water out of the shot
glass. Place another glass over the shot glass. This will keep air born
contaminates from settling in the shot glass while you wait for the shot
glass and water in the spore syringe to cool.
Fill the syringe with hot water from the mug. Eject the hot water and
repeat several times. This will insure the inside of the syringe and the
needle are clean and sterile. This is especially important if you are
using a syringe from a previous crop. When the needle is inserted into
the substrate, it is possible to get nutrients up inside the needle and for
contamination to grow. The last time you fill the syringe with hot
water, do not purge it. Let it sit in the syringe until it is cool. This is
useful for two reasons. First, the continued heat from the water can still
work to eliminate any remaining contaminates. Secondly, once the
water is cool it can be used as the sterile water needed to fill the
syringe. Make certain that nothing touches the needle of the syringe.
The Psilocybe mushroom spores will be killed if they come in contact
(9 of 15) 5/1/2002 6:54:20 PM]
How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 3)
with anything too hot. You need to wait until the shot glass and spore
syringe are at room temperature. When it is safe to proceed, use the
cigarette lighter to flame sterilize the X-Acto knife and the needle of
the syringe. Let the blade of the knife cool, but make sure it does not
touch anything. When it is cool, carefully open the spore print and
scrape a fleck of spores into the shot glass. A fleck 1/4 inch by 1/4 inch
is more than sufficient for a 10 cc. spore syringe. Use the needle of the
syringe to stir the spores into a few drops of water. Usually, there will
be a few drops left over in the shot glass from when you emptied it.
Otherwise, you can get the drops from the syringe. Stir the fleck of
spores until they are well broken up and 'dissolved' into the water.
Purge the water out of the syringe into the shot glass. Pull the water
back into the syringe, being sure to suck the spores in also. Do this
once or twice more to make sure the spore
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