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wnward through
the alumina, two zones that fluoresce blue can be spotted by
illumination with a black light. The faster-moving zone contains LSD,
while the slower-moving zone is iso-LSD.
When the zone containing LSD reaches the spigot of the burette, it
should be collected in a separate flask. About 3000 ml of the 3-1
benzene-chloroform is required to get the LSD moved down the
chromatography column, and finally eluted.
The iso-LSD is then flushed from the column by switching the
solvent being fed into the top of the column to chloroform. This
material is collected in a separate flask, and the solvent removed
under a vacuum. The residue is iso-LSD, and should be stored in the
freezer until conversion to LSD is undertaken. Directions for this are
also given in this chapter.
For the fraction containing the LSD, conversion to LSD tartrate
must be done to make it water soluble, improve its keeping
characteristics, and to allow crystallization. Tartaric acid has the
ability to react with two molecules of LSD. Use, then, of a 50% excess of
tartaric acid dictates the use of about 1 gram of tartaric acid to 3
grams of LSD. The three grams of LSD would be expected from a
well-done batch out of a total 3.5 LSD/iso-LSD mix.
The crystalline tartrate is made by dissolving one gram of tartaric
acid in a few mis of methanol, and adding this acid solution to the
benzene-chloroform elute from the chromatography column.
Evaporation of the solvent to a low volume under a vacuum gives
crystalline LSD tartrate. Crystals are often difficult to obtain. Instead,
an oil may result due to the presence of impurities. This is not cause
for alarm; the oil is still likely 90%+ pure. It should be bottled up in
dark glass, preferably under a nitrogen atmosphere, and kept in a
freezer until moved.
If chromatography reveals that one's chosen cooking method
produces little of the iso products, then the production of the tartrate
salt and crystallization is simplified. The residue obtained at the end
Practical LSD Manufacture
32
of the batch is dissolved in a minimum amount of methanol. To this is
then added tartaric acid. The same amount is added as above: one gram
tartaric acid to three grams LSD. Next, ether is slowly added with
vigorous stirring until a precipitate begins to form. The stoppered flask is
then put in the freezer overnight to complete the precipitation. After
filtering or centrifuging to isolate the product, it is transferred to a dark
bottle, preferably under nitrogen, and kept in the freezer until moved.
LSD from (so-LSD
Two variations on this procedure will be presented here. The first is
the method of Smith and Timmis from The Journal of the
Chemistry Society Volume 139, H pages 1168-1169 (1936). The other is
found in US patent 2,736,728. Both use the action of a strong
hydroxide solution to convert iso material into a mixture that contains
active and iso material. At equilibrium, the mixture contains about 2/3
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to accomplish.
Back to Method 3 of Terrarium Construction.
table of contents.
Adaptation-20: Providing a Moisture Reservoir in the
Substrate
A reservoir for moisture can be built into the cake while packing the
jars with substrate material. Start with an empty jar and tamp 1/4 inch
of substrate material in the bottom. Next, position a magic marker or
some other object with a cylindrical shape and a diameter of about 3/4
inch in the center of the jar. Fill the jar as normal. Next, remove the
object and fill the bore with damp vermiculite. Lastly, pack 1/4 inch
of substrate material over the entire jar, sealing the damp vermiculite
inside the cake.
The damp vermiculite can be mixed separately from the substrate
material. Use about 1 cup of vermiculite and 1/3 cup of water. This
should produce enough damp vermiculite for a dozen 1/2 pint culture
jars.
After the cake has fruited, occasionally inject water into this
reservoir. The easiest place to inject is the position of the cake that
used to be at the center of the bottom of the jar. Just insert a syringe
filled with sterile water and inject water until it is full.
In case you are wondering, the substrate material can't be injected
with water directly. The rice flour and vermiculite form a very tight
seal and it is next to impossible to inject water. Even if you do
(30 of 39) 5/1/2002 6:54:26 PM]
How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 4)
manage to inject water, it doesn't spread through the cake. Having this
reservoir filled with damp vermiculite gives the mycelium network
access to a lot of moisture.
The easiest way we have found to implement this adaptation is to cut
the bottom off of a 10 cc syringe and press the bottom of the syringe
into the wet vermiculite several times. This will load the syringe with
wet vermiculite. Then place the syringe in the culture jar and pack the
substrate around it. Use the syringe's plunger to hold the wet
vermiculite stationary while you remove the syringe. Tamp an
additional 1/4 inch of substrate material over the entire surface of the
jar.
Advantage:
This adaptation will dramatically extend the longevity of the cake.
The cake's yield are normally limited by the amount of moisture in
the cake. This procedure allows you to replace moisture as it is
consumed by mushroom growth. In general, you will double the total
output of the cake compared to not implementing this adaptation.
Disadvantages:
It is a little bit of extra work to prepare the jars this way. Also, there is
a need to be careful to use sterile water when injecting moisture
during the growing phase. There is no way to check for contamination
introduced inside the cake by injecting water.
Back to preparation and colonization
table of contents.
Adaptation-21: Use of a Timer to Control Suspended Water
Droplets and Stimulate Fruiting
The ultra sonic humidifier can be placed on a timer to control the
amount of suspended water
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